Targeting cell surface glycans with lectin-coated fluorescent nanodiamonds
Mina Ghanimi Fard, Zahra Khabir, Philipp Reineck, Nicole M. Cordina, Hiroshi Abe, Takeshi Ohshima, Sagar Dalal, Brant C. Gibson, Nicolle H. Packer, Lindsay M. Parker
Abstract
lectin (AAL). The lectin conjugated nanodiamonds were taken up differently by the various brain cell types with fucose binding AAL/FNDs taken up preferentially by glioblastoma phenotype astrocyte cells (U87-MG), sialic acid binding WGA/FNDs by neuronal phenotype cells (PC12) and high mannose binding TL/FNDs by microglial cells (BV-2). With increasing recognition of glycans having a role in many diseases, the lectin bioconjugated nanodiamonds developed here are well suited for further investigation into theranostic applications.
Topics & Concepts
FluorescenceGlycanLectinCellBiophysicsNanotechnologyChemistryMaterials scienceBiochemistryGlycoproteinBiologyPhysicsQuantum mechanicsDiamond and Carbon-based Materials ResearchPorphyrin and Phthalocyanine ChemistryCarbon Nanotubes in Composites