Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
Razan Sheta, Maxime Teixeira, Walid Idi, Abid Oueslati
Abstract
Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).1
Topics & Concepts
Induced pluripotent stem cellDopaminergicHuman Induced Pluripotent Stem CellsNeuroscienceBiologyDopamineTranscription factorStem cellNeural stem cellCell biologyEmbryonic stem cellGeneGeneticsPluripotent Stem Cells ResearchNeuroscience and Neural EngineeringCRISPR and Genetic Engineering