Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells
Kristin Roseth Aass, Robin Mjelle, Martin H. Kastnes, Synne Stokke Tryggestad, Luca M. van den Brink, Ingrid Aass Roseth, Marita Westhrin, Muhammad Zahoor, Siv Helen Moen, Tonje Marie Vikene Nedal, Glenn Buene, Kristine Misund, Anne‐Marit Sponaas, Qianli Ma, Anders Sundan, Richard W.J. Groen, Tobias S. Slørdahl, Anders Waage, Therese Standal
Abstract
. High-throughput transcriptomic and MS-metabolomic profiling of IL-32 KO cells revealed that cells depleted of IL-32 had perturbations in metabolic pathways, with accumulation of lipids, pyruvate precursors, and citrate. IL-32 was expressed in a subgroup of myeloma patients with inferior survival, and primary myeloma cells expressing IL-32 had a gene signature associated with immaturity, proliferation, and oxidative phosphorylation. In conclusion, we demonstrate a previously unrecognized role of IL-32 in the regulation of plasma cell metabolism.