A Bioinspired Five‐Coordinated Single‐Atom Iron Nanozyme for Tumor Catalytic Therapy
Bolong Xu, Shanshan Li, Lirong Zheng, Yunhang Liu, Along Han, Jin Zhang, Zhijun Huang, Haijiao Xie, Kelong Fan, Lizeng Gao, Huiyu Liu
Abstract
Abstract Single‐atom nanozymes (SAzymes) represent a new research frontier in the biomedical fields. The rational design and controllable synthesis of SAzymes with well‐defined electronic and geometric structures are essential for maximizing their enzyme‐like catalytic activity and therapeutic efficacy but remain challenging. Here, a melamine‐mediated pyrolysis activation strategy is reported for the controllable fabrication of iron‐based SAzyme containing five‐coordinated structure (FeN 5 ), identified by transmission electron microscopy imaging and X‐ray absorption fine structure analyses. The FeN 5 SAzyme exhibits superior peroxidase‐like activity owing to the optimized coordination structure, and the corresponding catalytic efficiency of Fe‐species in the FeN 5 SAzyme is 7.64 and 3.45 × 10 5 times higher than those in traditional FeN 4 SAzyme and Fe 3 O 4 nanozyme, respectively, demonstrated by steady‐state kinetic assay. In addition, the catalytic mechanism is jointly disclosed by experimental results and density functional theory studies. The as‐synthesized FeN 5 SAzyme demonstrates significantly enhanced antitumor effect in vitro and in vivo due to the excellent peroxidase‐like activity under tumor microenvironment.