Glycan-Gold Nanoparticles as Multifunctional Probes for Multivalent Lectin–Carbohydrate Binding: Implications for Blocking Virus Infection and Nanoparticle Assembly
Darshita Budhadev, Emma Poole, Inga Nehlmeier, Yuanyuan Liu, James Hooper, Elizabeth Kalverda, Uchangi Satyaprasad Akshath, Nicole Hondow, W. Bruce Turnbull, Stefan Pöhlmann, Yuan Guo, Dejian Zhou
Abstract
values down to 95 pM), but only partially block DC-SIGNR-mediated virus infection. Our results suggest that the ability of a glycoconjugate to simultaneously block all binding sites of a target lectin is key to robust inhibition of viral infection.
Topics & Concepts
GlycanChemistryGlycoconjugateIsothermal titration calorimetryLectinBiophysicsBiochemistryReceptor–ligand kineticsPlasma protein bindingBinding siteGlycoproteinReceptorBiologyBacteriophages and microbial interactionsImmunotherapy and Immune ResponsesMonoclonal and Polyclonal Antibodies Research