Yearlong COVID-19 Infection Reveals Within-Host Evolution of SARS-CoV-2 in a Patient With B-Cell Depletion
Véronique Nussenblatt, Allison Roder, Sanchita Basu Das, Emmie de Wit, Jung-Ho Youn, Stephanie Banakis, Alexandra A. Mushegian, Christopher Mederos, Wei Wang, Matthew Chung, Lizzette Pérez-Pérez, Tara N. Palmore, Jennifer N. Brudno, James N. Kochenderfer, Elodie Ghedin
Abstract
B-cell-depleting therapies may lead to prolonged disease and viral shedding in individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and this viral persistence raises concern for viral evolution. We report sequencing of early and late samples from a 335-day infection in an immunocompromised patient. The virus accumulated a unique deletion in the amino-terminal domain of the spike protein, and complete deletion of ORF7b and ORF8, the first report of its kind in an immunocompromised patient. Unique viral mutations found in this study highlight the importance of analyzing viral evolution in protracted SARS-CoV-2 infection, especially in immunosuppressed hosts.