The Reactome Knowledgebase 2026
Eliot Ragueneau, Chuqiao Gong, Pierre Sinquin, Cristoffer Sevilla, Deidre Beavers, Alexander Grentner, Johannes Griss, Gregory Hogue, Nancy T. Li, Lisa Matthews, Bruce May, M Orlic-Milacic, Helia Mohammadi, Robert Petryszak, Karen Rothfels, Veronica Shamovsky, Ralf Stephan, Krishna Kumar Tiwari, Joel Weiser, Adam Wright, Marc Gillespie, Guanming Wu, Lincoln Stein, Henning Hermjakob, Peter D’Eustachio
Abstract
The Reactome Knowledgebase (https://reactome.org) is a freely accessible, expert-curated, open-source, and open-data resource that describes human biology in molecular detail. It spans normal physiology as well as disease mechanisms, including the impact of genetic variation and drug action. Reactome content is continuously expanded and revised, with automated workflows now monitoring retracted publications to maintain data integrity. To meet the needs of a growing user base, Reactome has launched a redesigned Angular-based interface with enhanced accessibility, modular architecture, and a hierarchy of visualization tools: ReacFoam for global pathway overviews, enhanced high-level diagrams for intuitive navigation, and redesigned entity level views (ELVs) enriched with chemical structures, animated protein models, and a new "compare mode" to contrast normal and disease states. New analysis tools support multi-omics integration and customizable visualizations. Recent innovations include the React-to-me chatbot for natural language interaction, community-driven tutorials, and an open Figma icon library. Reactome's sustainability and compliance with FAIR data principles were recently recognized with CoreTrustSeal certification and its designation as a Global Core Biodata and ELIXIR resource, reinforcing its role as a trusted global knowledgebase.