Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana
Huacai Fan, Ping He, Shengtao Xu, Shu Li, Yongfen Wang, Wenlong Zhang, Xundong Li, Hui Shang, Li Zeng, Si-Jun Zheng
Abstract
Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 ( Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter , Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen ( Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43–81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants.