Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides
Muhan He, Xunshen Wu, Song Mao, Phensinee Haruehanroengra, Irfan Khan, Jia Sheng, Maksim Royzen
Abstract
Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.
Topics & Concepts
OligonucleotideSolid-phase synthesisRNAHigh-performance liquid chromatographyChemistryPhase (matter)Combinatorial chemistryChromatographyBiochemistryOrganic chemistryDNAGenePeptideRNA and protein synthesis mechanismsRNA Interference and Gene DeliveryRNA modifications and cancer