A novel dual-function SERS-based identification strategy for preliminary screening and accurate diagnosis of circulating tumor cells
Dinghu Zhang, Jie Lin, Yanping Xu, Xiaoxia Wu, Xiawei Xu, Yujiao Xie, Ting Pan, Yiwei He, Jun Luo, Zhewei Zhang, Linyin Fan, Shunxiang Li, Tianxiang Chen, Aiguo Wu, Guoliang Shao
Abstract
-Au complex SERS bioprobe was developed, which could effectively capture the triple negative breast cancer (TNBC) cells and endow the tumor cells with distinct SERS signals. Then, the ROC curve obtained based on the comparison of SERS intensity of TNBC cells and WBC was used to construct a tumor cell identification model. The merit of the model was that the detection sensitivity and specificity could be intelligently switched according to different identification purposes such as accurate diagnosis or preliminary screening of tumor cells. Finally, the difunctional recognition ability of the model for accurate diagnosis and preliminary screening of tumor cells was further validated by using the healthy human blood added with TNBC cells and blood samples of real tumor patients. This novel difunctional identification strategy provides a new perspective for identification of CTCs based on the SERS technology.