Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study
Poulami Mandal, Jérôme Kretzschmar, Björn Drobot
Abstract
The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good's buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good's buffers.
Topics & Concepts
ChemistryEuropiumLanthanideTrisPiperazineMorpholineInorganic chemistryHEPESIonFluorescenceBuffer solutionNuclear magnetic resonance spectroscopySpectroscopyPhysical chemistryStereochemistryOrganic chemistryPhysicsQuantum mechanicsBiochemistryLanthanide and Transition Metal ComplexesRadioactive element chemistry and processingElectrochemical Analysis and Applications