Real-time monitoring of the sialic acid biosynthesis pathway by NMR
Jacob L. Gorenflos López, Peter Schmieder, Kristin Kemnitz‐Hassanin, Hatice Ceyda Asikoglu, Arif Celik, Christian E. Stieger, Dorothea Fiedler, Stephan Hinderlich, Christian P. R. Hackenberger
Abstract
From chemical shifts to conversion velocity: real-time NMR spectroscopy using a combination of 1D and 2D experiments can monitor individual enzymatic steps in the sialic acid biosynthesis pathway in rat liver cytosolic extract.
Topics & Concepts
BiochemistrySialic acidBiosynthesisCytosolEnzymeKinasePhosphorylationN-AcetylglucosamineSialyltransferaseGlycoproteinChemistryBiologyGlycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisBiochemical and Molecular Research