A sensitive and specific method for microRNA detection and <i>in situ</i> imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
Yang Liu, Shihong Li, Likun Zhang, Qian Zhao, Nuo Li, Yuxin Wu
Abstract
We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly.
Topics & Concepts
CRISPRCas9In situmicroRNAMicrovesiclesComputational biologyNanotechnologyDetection limitChemistryBiologyMaterials scienceGeneChromatographyBiochemistryOrganic chemistryAdvanced biosensing and bioanalysis techniquesExtracellular vesicles in diseaseCRISPR and Genetic Engineering