Differential photosynthetic responses to drought stress in peanut varieties: insights from transcriptome profiling and JIP-Test analysis

Abstract

Abstract Background Drought stress poses a critical constraint to plant growth by impairing photosynthetic efficiency in crops. Results Through transcriptome profiling of two peanut cultivars with contrasting drought tolerance, Fuhua18 (drought-sensitive, FH18) and Nonghua5 (drought-tolerant, NH5), we identified significant enrichment of differentially expressed genes in photosynthesis-related pathways. Notably, these genes were predominantly downregulated in FH18. Subsequent physiological analysis revealed cultivar-specific responses: Chlorophyll content decreased in FH18 but increased in NH5 after 24 h of drought treatment, accompanied by significant reductions in net photosynthetic rate (Pn) and water use efficiency (WUE) in both cultivars. The drought-induced physiological perturbations were further evidenced by elevated electrolyte leakage and activated antioxidant systems. To dissect photosynthetic apparatus dynamics, we implemented JIP-test analysis of chlorophyll fluorescence kinetics. Both cultivars exhibited substantial increases in Vj and Vi parameters at 24 h, while FH18 demonstrated a pronounced elevation in Vk during the O-J phase transition, suggesting severe impairment of the oxygen-evolving complex. Quantitative evaluation of photosynthetic performance indices revealed marked declines in PI abs and PI total , indicating systemic damage to both PSI and PSII under drought stress. Comparative analysis identified 11 traits showing significant inter-cultivar variation, particularly in PSII reaction center parameters including PI abs , DI 0 /RC, RE 0 /RC, ABS/RC, and TR 0 /RC. Conclusion These findings provide mechanistic insights into cultivar-dependent photosynthetic responses to drought stress, offering potential biomarkers for breeding drought-resilient peanut varieties.

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