Er<sup>3+</sup>-to-dye energy transfer in DNA-coated core and core/shell/shell upconverting nanoparticles with 980 nm and 808 nm excitation of Yb<sup>3+</sup>and Nd<sup>3+</sup>
Laura Francés‐Soriano, Nicola Peruffo, Marta Maria Natile, Niko Hildebrandt
Abstract
), all UCNP-DNA nanohybrids showed very similar concentration dependent FRET-quenching of UCNP luminescence with efficiencies between 0 and ∼20%. We analyzed luminescence intensities, decay times, and rise times and could show the entanglement of excitation and emission kinetics by simply changing the excitation wavelength from 980 nm to 808 nm for the same css-UCNPs. Time-gated FRET-sensitized dye luminescence showed dye-ssDNA concentration dependence over four orders of magnitude (1 nM to 10 μM), which suggested a possible application to nucleic acid biosensing for both 808 and 980 nm excitation.
Topics & Concepts
ExcitationPhoton upconversionNanoparticleCore (optical fiber)Energy transferShell (structure)Förster resonance energy transferMaterials scienceFluorescenceNanotechnologyChemistryMolecular physicsPhysicsOpticsComposite materialQuantum mechanicsAdvanced biosensing and bioanalysis techniquesLuminescence and Fluorescent MaterialsQuantum Dots Synthesis And Properties