Regulation of microglia polarization <i>via</i> mannose receptor-mediated delivery of siRNA by ligand-functionalized DoGo LNP
Hai Xiao, Shuqin Han, Huricha Baigude
Abstract
. We confirmed that siTLR4/M3 complexes were specifically internalized by BV2 cells in a MR-dependent manner, and the treatment of oxygen glucose deprivation (OGD)-treated BV2 cells with siTLR4/M3 complexes resulted in remarkable silencing of TLR4, and induced downregulated M1 polarization and upregulated M2 polarization markers. Collectively, our data suggest that the M3 lipoplex is a promising microglia-targeting siRNA delivery agent.
Topics & Concepts
Mannose receptorMicrogliaDownregulation and upregulationNeuroprotectionChemistryInternalizationTLR4MannoseReceptorCell biologyGene silencingIn vitroPharmacologyInflammationBiochemistryImmunologyBiologyGeneMacrophageRNA Interference and Gene DeliveryNeuroinflammation and Neurodegeneration MechanismsImmune Response and Inflammation