Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search
Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Junjie Liu, Chunlai Chen
Abstract
Nonspecific interactions between DNA ∼8 bp downstream of the PAM and lysines within residues 1151–1156 of Cas9 mediate one-dimensional diffusion and cause asymmetric target search regions flanking the PAM.
Topics & Concepts
Downstream (manufacturing)DiffusionChemistryCell biologyComputational biologyBiologyPhysicsEngineeringOperations managementThermodynamicsCRISPR and Genetic EngineeringRNA regulation and diseaseRNA and protein synthesis mechanisms