Reannotation of Fly Amanita <scp>l</scp>-DOPA Dioxygenase Gene Enables Its Cloning and Heterologous Expression
Douglas M. M. Soares, Letícia Christina Pires Gonçalves, Caroline Oliveira Machado, Larissa Cerrato Esteves, Cassius V. Stevani, Carla C. Oliveira, Felipe Augusto Dörr, Ernani Pinto, Flávia M. M. Adachi, Carlos Takeshi Hotta, E. L. Bastos
Abstract
folding using deep learning algorithms, and their putative active sites were inferred and compared. The wide catalytic pocket of AmDODA and the presence of the His-His-His and His-His-Asp motifs can provide insight into the dual cleavage of l-DOPA at positions 2,3 and 4,5 as per the mechanism proposed for nonheme dioxygenases.
Topics & Concepts
Heterologous expressionDioxygenaseBiologyGenePlasmidHeterologousComplementary DNACloning (programming)cDNA libraryMolecular biologyGeneticsRecombinant DNAComputer scienceProgramming languageBotanical Research and ApplicationsChemical synthesis and alkaloidsMicrobial Metabolism and Applications