α-Galactosylceramide-Reactive NKT Cells Increase IgG1 Class Switch against a Clostridioides difficile Polysaccharide Antigen and Enhance Immunity against a Live Pathogen Challenge
Gillian A. Lang, Binu Shrestha, Souwelimatou Amadou Amani, Tyler M. Shadid, Jimmy D. Ballard, Mark L. Lang
Abstract
mice were not protected by the PSII/α-GC immunization modality. Absence of NKT cells similarly had a modest effect on isotype switch, but ratios of IgG1/IgG2c decreased. These results indicate that α-GC-driven NKT cells move the humoral immune response against C. difficile PSII antigen toward Th2-driven IgG1 and may contribute to augmented protection. This study suggests that NKT activation represents a pathway for additional B-cell help that could be used to supplement existing efforts to develop vaccines against polysaccharides derived from C. difficile and other pathogens.
Topics & Concepts
BiologyMicrobiologyCD1DAntigenIsotypeImmune systemAntibodyImmunologyNatural killer T cellT cellMonoclonal antibodyClostridium difficile and Clostridium perfringens researchImmune Cell Function and Interaction