A Golgi-targeted viscosity rotor for monitoring early alcohol-induced liver injury
Chuanhao Liu, Lin Zhou, Ying Zheng, Huizi Man, Zhiwei Ye, Xinfu Zhang, Lijuan Xie, Yi Xiao
Abstract
We proposed to monitor the early stage of alcohol-induced liver injury through quantitatively detecting Golgi viscosity. Therefore, the first Golgi-targeted fluorescent rotor (GA-Vis) was developed. With the aid of GA-Vis, the changes in Golgi viscosity during alcohol-induced liver injury were quantitatively evaluated by fluorescence lifetime imaging in live cells and zebrafish. GA-Vis was qualified as a practical tool for future diagnoses of alcohol-induced liver injury.
Topics & Concepts
Golgi apparatusLiver injuryViscosityAlcoholRotor (electric)ZebrafishFluorescenceChemistryCell biologyBiophysicsMaterials scienceBiomedical engineeringMedicineBiologyBiochemistryPhysicsGeneCellInternal medicineOpticsQuantum mechanicsComposite materialEndoplasmic Reticulum Stress and DiseaseAdvanced Glycation End Products researchAlcohol Consumption and Health Effects