Catalytic hairpin assembly-based electrochemical biosensor with tandem signal amplification for sensitive microRNA assay
Lin Cui, Jinghua Zhou, Xiaoyun Yang, Jing Dong, Xiaolei Wang, Chun‐yang Zhang
Abstract
We demonstrate for the first time the construction of a low background electrochemical biosensor with tandem signal amplification for sensitive microRNA assay based on target-activated catalytic hairpin assembly (CHA) of heteroduplex-templated copper nanoparticles. This electrochemical biosensor exhibits high sensitivity, good specificity, single-base mismatch discrimination capability, excellent stability and reproducibility, and it can sensitively detect microRNA in cancer cells.
Topics & Concepts
BiosensorTandemChemistryElectrochemistrymicroRNACombinatorial chemistryCatalysisSIGNAL (programming language)NanotechnologyChromatographyMaterials scienceElectrodeBiochemistryComputer sciencePhysical chemistryComposite materialProgramming languageGeneAdvanced biosensing and bioanalysis techniquesMicroRNA in disease regulationRNA Interference and Gene Delivery