The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b
Evan Chaudhuri, Sabyasachi Dash, Muthukumar Balasubramaniam, Adrian Padron, Joseph Holland, Gregory A. Sowd, Fernando Villalta, Alan Engelman, Jui Pandhare, Chandravanu Dash
Abstract
3'UTR. Interestingly, HIV-1 infection down-regulated miR-125b expression concurrent with up-regulation of CPSF6. Notably, miR-125b down-regulation in infected cells was not due to reduced pri-miRNA or pre-miRNA levels. However, miR-125b down-regulation depended on HIV-1 reverse transcription but not viral DNA integration. These findings establish a post-transcriptional mechanism that controls CPSF6 expression and highlight a novel function of miR-125b during HIV-host interaction.
Topics & Concepts
microRNAThree prime untranslated regionBiologyCell biologyGene knockdownPolyadenylationUntranslated regionCleavage and polyadenylation specificity factorTranscription factorMessenger RNAGeneticsGeneRNA Research and SplicingRNA modifications and cancerGenomics and Chromatin Dynamics