DNA labelling in live cells <i>via</i> visible light-induced [2+2] photocycloaddition
Rita T. Michenfelder, Laura Delafresnaye, Vinh X. Truong, Christopher Barner‐Kowollik, Hans‐Achim Wagenknecht
Abstract
the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.
Topics & Concepts
LabellingMoietyVisible spectrumDNAFluorescenceHeLaChemistryCycloadditionPhotochemistryBODIPYFluorescent lightBiophysicsCellBiochemistryStereochemistryOptoelectronicsBiologyMaterials scienceOpticsCatalysisPhysicsClick Chemistry and ApplicationsChemical Synthesis and AnalysisPhotochromic and Fluorescence Chemistry