910 metagenome-assembled genomes from the phytobiomes of three urban-farmed leafy Asian greens
Aditya Bandla, Shruti Pavagadhi, Ashwin Sridhar Sudarshan, Miko Chin Hong Poh, Sanjay Swarup
Abstract
The genome sequences of many microbial species from the phytobiomes of several leafy Asian greens remain unknown. Here, we address this gap by reconstructing 910 prokaryotic draft genomes from 24 leaf, 65 root, 12 soil, and 6 compost metagenomes from the seedling and adult developmental stages of three leafy Asian greens - Brassica rapa var. parachinensis, Brassica oleracea var. alboglabra and Amaranthus spp. - grown in a commercial, soil-based urban farm. Of these, 128 are near-complete (>90% completeness, <5% redundancy), 540 are substantially complete (≥70% completeness, <10%, redundancy), while the rest have a completeness ≥50% and redundancy <10%. The draft genomes together span 292 bacterial and 3 archaeal species, a subset of which are from underrepresented genus-level lineages in public databases. We expect our dataset to facilitate a wide range of comparative studies that seek to understand the different functional aspects of vegetable crop phytobiomes and for devising new strategies for microbial cultivation in the future.