Carboxamide and N-alkylcarboxamide additives can greatly reduce non specific amplification in Loop-Mediated Isothermal Amplification for Foot-and-Mouth disease Virus (FMDV) using Bst 3.0 polymerase
Dua Mohammad Ghaith, Robin Abu Ghazaleh
Topics & Concepts
Loop-mediated isothermal amplificationReverse transcriptaseFoot-and-mouth disease virusBiologyRNAComplementary DNAVirusSYBR Green IMolecular biologyVirologyRecombinase Polymerase AmplificationRNA-Directed DNA PolymeraseReverse Transcription Loop-mediated Isothermal AmplificationReal-time polymerase chain reactionDNABiochemistryGeneAnimal Disease Management and EpidemiologyViral Infections and Immunology ResearchAnimal Virus Infections Studies