Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances
László Hajba, Sunkyung Jeong, Doo Soo Chung, András Guttman
Abstract
This review summarizes the fundamental principles, basic methodologies, strength and weaknesses of capillary gel electrophoresis of proteins by providing both a short historical overview and highlighting new developments and applications in biopharmaceutical, biomedical as well as food and agriculture fields. The subsets of the method including native capillary gel electrophoresis, SDS capillary gel electrophoresis, capillary gel isoelectric focusing, capillary gel isotachophoresis and capillary affinity gel electrophoresis of proteins are all critically reviewed. Relevant protein labeling techniques are also addressed.
Topics & Concepts
IsotachophoresisCapillary electrophoresisIsoelectric focusingFree-flow electrophoresisChromatographyGel electrophoresisGel electrophoresis of proteinsElectrophoresisTwo-dimensional gel electrophoresisChemistryCapillary actionCapillary electrochromatographyAffinity electrophoresisPolyacrylamide gel electrophoresisProteomicsMaterials scienceBiochemistryElectrolyteAffinity chromatographyComposite materialPhysical chemistryEnzymeGeneElectrodeMicrofluidic and Capillary Electrophoresis ApplicationsProtein purification and stabilityInnovative Microfluidic and Catalytic Techniques Innovation