Sox9EGFP Defines Biliary Epithelial Heterogeneity Downstream of Yap Activity
Deepthi Y. Tulasi, Diego Martinez Castaneda, Kortney Wager, Connor B. Hogan, Karel P. Alcedo, Jesse R. Raab, Adam D. Gracz
Abstract
BACKGROUND & AIMS: transgene in the liver and demonstrate that green fluorescent protein (GFP) expression levels are associated with distinct cell types. METHODS: mice were subjected to bile duct ligation, and GFP expression was assessed by immunofluorescence. RESULTS: remains restricted to BECs and periportal hepatocytes after bile duct ligation. CONCLUSIONS: levels provide readout of Yap activity and delineate BEC heterogeneity, providing a tool for assaying subpopulation-specific cellular function in the liver.
Topics & Concepts
OrganoidGreen fluorescent proteinBiologyImmunofluorescenceCell biologyFlow cytometryHES1Bile ductTransgeneTransfectionGenetically modified mouseProximity ligation assayCell sortingMolecular biologyCell cultureSignal transductionGeneNotch signaling pathwayImmunologyReceptorGeneticsInternal medicineAntibodyMedicineLiver physiology and pathologyHippo pathway signaling and YAP/TAZWnt/β-catenin signaling in development and cancer