A 3D cell printing-fabricated HepG2 liver spheroid model for high-content <i>in situ</i> quantification of drug-induced liver toxicity
Sera Hong, Joon Myong Song
Abstract
values. In addition, 3D HepG2 spheroids were more resistant to nefazodone-induced MPT than 2D HepG2 cells, indicating that the gelatin/alginate hydrogel culture system provides enhanced resistance to hepatotoxic drugs. The drug response of HepG2 liver spheroids was also found to be unrelated to the spheroid size. These results demonstrate that the present 3D cell-printing-based embedded HepG2 liver spheroid platform is a promising approach for screening and characterizing drug-induced hepatotoxicity.
Topics & Concepts
SpheroidIn situToxicityLiver toxicityDrugLiver tissueChemistryLiver cellPharmacologyMedicineIn vitroBiochemistryInternal medicineOrganic chemistry3D Printing in Biomedical ResearchAdditive Manufacturing and 3D Printing TechnologiesInnovative Microfluidic and Catalytic Techniques Innovation