Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors
Sandra Burgstaller, Teresa R. Wagner, Helmut Bischof, Sarah Bueckle, Aman Padamsey, Desiree I. Frecot, Philipp D. Kaiser, David Skrabak, Roland Malli, Robert Łukowski, Ulrich Rothbauer
Abstract
and pH sensors, which specifically stained HER2-positive breast cancer cells. Based on the successful development of several Nb-fused biosensor combinations, we anticipate that this approach can be readily extended to other biosensors and will open new opportunities for the study of extracellular analytes in advanced experimental settings.
Topics & Concepts
BiosensorExtracellularAnalyteChemistryCancer cellFörster resonance energy transferTumor microenvironmentBiophysicsBiochemistryNanotechnologyCancerFluorescenceBiologyMaterials scienceChromatographyQuantum mechanicsGeneticsPhysicsMonoclonal and Polyclonal Antibodies ResearchAdvanced Biosensing Techniques and ApplicationsGlycosylation and Glycoproteins Research