A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2
José Carlos Ponce‐Rojas, Michael S. Costello, Duncan A. Proctor, Kenneth S. Kosik, Maxwell Z. Wilson, Carolina Arias, Diego Acosta‐Alvear
Abstract
) that addresses this limitation. PEARL uses a lysis solution that disrupts cell membranes and viral envelopes while simultaneously providing conditions suitable for alcohol-based precipitation of RNA, DNA, and proteins. PEARL is a fast, low-cost, and simple method that uses common laboratory reagents and offers performance comparable to that of commercial RNA extraction kits. PEARL offers an alternative method to isolate host and pathogen nucleic acids and proteins to streamline the detection of DNA and RNA viruses, including SARS-CoV-2.
Topics & Concepts
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Isolation (microbiology)RNAVirologyDNACoronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakComputational biologyBiologyRNA extractionGeneticsMedicineBioinformaticsGeneInfectious disease (medical specialty)OutbreakPathologyDiseaseViral gastroenteritis research and epidemiologySARS-CoV-2 detection and testingBiosensors and Analytical Detection