Transcriptome and phosphoproteomics provides potential insights into how sucrose regulates the growth of bamboo shoots
Huifang Zheng, Yucong Bai, Junlei Xu, Yali Xie, Zhanchao Cheng, Jian Gao
Abstract
Carbohydrates are vital for the growth and development of bamboo shoots; however, the molecular mechanism by which sucrose affects the growth and development of bamboo shoots remains unclear. In this study, the application of exogenous sucrose at the early stage of bamboo shoot growth promoted rapid growth. Transcriptome sequencing indicated that sucrose primarily regulates the growth of bamboo shoots through starch and sucrose metabolism and auxin signal transduction. A phosphoproteome analysis revealed that the phosphorylation state of 297 proteins significantly changed following sucrose treatment, including key proteins involved in sugar metabolism. Sucrose nonfermenting protein kinase PheSnRK1.4 and PheSnRK1.6 were upregulated in darkness and overexpression of PheSnRK1.6 in A. thaliana plants resulted in higher growth compared with that of empty carrier A. thaliana . Yeast one-hybrid experiments revealed that the early auxin signal factors, PheIAA4.2 and PheIAA16.3, can bind to the promoter of PheSnRK1.6 . Sugar and auxin jointly regulate the growth and development of bamboo shoots, whereas PheSnRK1.6 may play an important role in this process. This study provides insight into the regulatory mechanisms of energy metabolism during bamboo shoot rapid growth. • Exogenous sucrose promotes early bamboo shoot growth via starch/sucrose metabolism and auxin signaling. • Sucrose alters protein phosphorylation linked to glucose metabolism, influencing bamboo shoot growth. • Auxin early response factor IAA regulates bamboo growth by modulating PheSnRK1.6 gene expression.