Role of Cytocentrifugation Combined with Nuclear Fast Picroindigocarmine Staining in Detecting Cryptozoospermia in Men Diagnosed with Azoospermia
Rakesh Sharma, Sajal Gupta, Ashok Agarwal, Renata Finelli, Shinnosuke Kuroda, Ramadan Saleh, Florence Boitrelle, Parviz K. Kavoussi, Murat Gül, Nicholas N. Tadros, Edmund Ko, Ala’a Farkouh, Ralf Henkel, Mohamed Arafa, Amarnath Rambhatla, Rupin Shah
Abstract
PURPOSE: Azoospermia is defined as the absence of spermatozoa in the pellet of a centrifuged semen sample. In fact, when a basic semen analysis fails to detect sperm in the ejaculate, there is still the possibility of detecting rare sperm after centrifugation of the sample and examination of the pellet. In this study, we assessed the role of Cytospin centrifugation in combination with the nuclear fast picroindigocarmine (NF-PIC) staining in identifying sperm in azoospermic samples. MATERIALS AND METHODS: Semen samples of 251 men diagnosed as having azoospermia after standard examination were further analyzed by Cytospin centrifugation in combination with NF-PIC staining. RESULTS: Sperm were detected in 60 men (23.9%), thus changing their diagnosis to cryptozoospermia. CONCLUSIONS: By identifying sperm in the semen of men who were thought to have total azoospermia, the Cytospin NF-PIC test can alter the diagnosis and further treatment of these men.