Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies
Yan Sheng, Zena Huang, Tenghua Zhang, Feiyang Qian, Yuqing Zhu, Zaizai Dong, Qingyang Zhang, Qi Lei, Fanhui Kong, Yuqiong Wang, Maddie Walden, Stefan Wuttke, Lingqian Chang, Wei Zhu, Jiaming Hu
Abstract
Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.
Topics & Concepts
ExosomeElectroporationChemistryMicrovesiclesmicroRNATransfectionRNACellCell biologyMessenger RNAComputational biologyBiochemistryGeneBiologyExtracellular vesicles in diseaseRNA Interference and Gene DeliveryNanopore and Nanochannel Transport Studies