Direct pathway cloning and expression of the radiosumin biosynthetic gene cluster
Xiaodan Ouyang, Paul M. D’Agostino, Matti Wahlsten, Endrews Delbaje, Jouni Jokela, Perttu Permi, Greta Gaiani, Antti Poso, Piia Bartos, Tobias A. M. Gulder, Hannu Koistinen, David P. Fewer
Abstract
values from 1.7 μM to >7.2 μM). These results provide a biosynthetic logic to explore the genetic and chemical diversity of the radiosumin family and suggest that these natural products may be a source of drug leads for selective human serine proteases inhibitors.
Topics & Concepts
Cloning (programming)Serine proteaseGene clusterSerineGeneProteaseBiologyGene expressionMolecular cloningTMPRSS6BiochemistryChemistryComputational biologyGeneticsCell biologyEnzymeComputer scienceProgramming languageAlgal biology and biofuel productionBiocrusts and Microbial EcologyAquatic Ecosystems and Phytoplankton Dynamics