A molecular analysis of meropenem–vaborbactam non-susceptible KPC-producing <i>Klebsiella pneumoniae</i>
Mohamad Yasmin, Steven H. Marshall, Liang Chen, Daniel D. Rhoads, Michael R. Jacobs, Laura J. Rojas, Federico Pérez, Andrea M. Hujer, Kristine M. Hujer, David van Duin, Vance G. Fowler, Henry F. Chambers, Barry N. Kreiswirth, Robert A. Bonomo, for the Antibacterial Resistance Leadership Group
Abstract
ABSTRACT We characterized the molecular determinants of meropenem–vaborbactam (MV) non-susceptibility among non-metallo-β-lactamase-producing KPC- Klebsiella pneumoniae (KPC- KP ). Whole-genome sequencing was performed to identify mutations associated with MV non-susceptibility. Isolates with elevated MV MICs were found to have mutations encoding truncated or altered OmpK36 porins and increased bla KPC copy numbers. KPC- KP isolates with decreased susceptibility to MV were detected among a collection of isolates predating the availability of MV.