Incarnatapeptins A and B, Nonribosomal Peptides Discovered Using Genome Mining and <sup>1</sup>H/<sup>15</sup>N HSQC-TOCSY
Kalindi D. Morgan, David E. Williams, Brian O. Patrick, Marion Remigy, Carmen A. Bañuelos, Marianne D. Sadar, Katherine S. Ryan, Raymond J. Andersen
Abstract
Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an 15 N NMR-based screening method for the targeted isolation of the low-abundance piperazic-acid-containing peptides incarnatapeptins A ( 1 ) and B ( 3 ). Incarnatapeptin B ( 3 ) shows in vitro cytotoxicity to LNCaP prostate cancer cells.
Topics & Concepts
LNCaPChemistryHeteronuclear single quantum coherence spectroscopyGenomeCytotoxicityDrug discoveryIsolation (microbiology)In vitroComputational biologyCombinatorial chemistryStereochemistryProstate cancerBiochemistryTwo-dimensional nuclear magnetic resonance spectroscopyCancerGeneGeneticsBiologyBioinformaticsMicrobial Natural Products and BiosynthesisRNA and protein synthesis mechanismsChemical Synthesis and Analysis