The <i>In Vivo</i> and <i>In Vitro</i> Architecture of the Hepatitis C Virus RNA Genome Uncovers Functional RNA Secondary and Tertiary Structures
Han Wan, Rebecca L. Adams, Brett D. Lindenbach, Anna Marie Pyle
Abstract
. In addition to providing a comprehensive atlas of functional secondary structural elements throughout the genomic RNA, we identified a novel set of tertiary interactions and demonstrated their functional importance. In terms of broader implications, the pipeline developed in this study can be applied to other long RNAs, such as long noncoding RNAs. In addition, the RNA structural motifs characterized in this study broaden the repertoire of known riboregulatory elements.
Topics & Concepts
BiologyRNAIn vitroIn vivoVirologyGenomeRNA-dependent RNA polymeraseRNA editingGeneGeneticsHepatitis C virus researchHepatitis B Virus StudiesRNA and protein synthesis mechanisms