<i>In Vivo</i> Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP<sup>+</sup>
Liliana Calzadiaz Ramirez, Carla Calvó‐Tusell, Gabriele Stoffel, Steffen N. Lindner, Sílvia Osuna, Tobias J. Erb, Marc Garcia‐Borràs, Arren Bar‐Even, Carlos G. Acevedo‐Rocha
Abstract
selection to identify highly proficient enzyme variants carrying multiple mutations which would be almost impossible to find using conventional screening methods.
Topics & Concepts
FormateFormate dehydrogenaseCofactorNAD+ kinaseEnzymeBiochemistryStereochemistryChemistryBiologyCombinatorial chemistryCatalysisEnzyme Catalysis and ImmobilizationAmino Acid Enzymes and MetabolismEnzyme Structure and Function