Effects of Cryopreservation on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Assessing Drug Safety Response Profiles
Joe Z. Zhang, Nadjet Belbachir, Tiejun Zhang, Yu Liu, Rajani Shrestha, Joseph C. Wu
Abstract
transients, and field potential duration. When subjected to treatment of drugs that affect electrophysiological properties, recovered hiPSC-CMs showed an altered drug response and enhanced propensity for drug-induced cardiac arrhythmic events. In conclusion, fresh and recovered hiPSC-CMs do not always show comparable molecular and physiological properties. When cryopreserved hiPSC-CMs are used for assessing drug-induced cardiac liabilities, the altered drug sensitivity needs to be considered.
Topics & Concepts
CryopreservationInduced pluripotent stem cellTranscriptomeBiologyDrugContractilityPharmacologyCell biologyGeneEmbryoGene expressionGeneticsEndocrinologyEmbryonic stem cellPluripotent Stem Cells ResearchNeuroscience and Neural Engineering3D Printing in Biomedical Research