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Pedobacter endophyticus sp. nov., an endophytic bacterium isolated from Carex pumila

Yuxin Peng, Lingmin Jiang, Jiyoon Seo, Zhun Li, Hanna Choe, Jae Cheol Jeong, Suk Weon Kim, Young‐Min Kim, Cha Young Kim, Jiyoung Lee

2021INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY10 citationsDOI

Abstract

An aerobic, Gram-stain-negative, weak-motile, short-rod-shaped bacterial strain, designated JBR3-12 T , was isolated from halophyte Carex pumila plants, and its taxonomic position was investigated by using a polyphasic taxonomic approach. The strain produced a pink pigment on tryptic soy agar and grew optimally at 25 °C, pH 8 and in the presence of 3 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR3-12 T formed a lineage within the genus Pedobacter and was most closely related to Pedobacter sandarakinus DS-27 T (98.0 %) and Pedobacter agri PB92 T (97.6 %). The DNA G+C content of the genome was 41.3 mol%; the whole genome length was 5 426 070 bp. The major fatty acids of JBR3-12 T were iso-C 15 : 0 , summed feature 3 (comprising C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c ) and iso-C 17 : 0 3-OH. The predominant polar lipid was phosphatidylethanolamine. The predominant quinone was menaquinone-7. Based on its phenotypic, phylogenetic and genotypic features, strain JBR3-12 T is proposed to represent a novel species of the genus Pedobacter , for which the name is Pedobacter endophyticus sp. nov. The type strain is JBR3-12 T (=KCTC 82363 T =NBRC 114901 T ).

Topics & Concepts

Biology16S ribosomal RNAPhylogenetic treeBotanyPhylogeneticsGenome sizeTaxonomy (biology)BacteriaStrain (injury)GenomeGeneGeneticsAnatomyGenomics and Phylogenetic StudiesBacterial Identification and Susceptibility TestingMicrobial Natural Products and Biosynthesis
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