Oxygen Self‐Supplying Enzymatic Nanoplatform for Precise and Enhanced Photodynamic Therapy
Xiaohui Wang, Xiao-Fei Wei, Jinhua Liu, Dan He, Dandan Sun, Wei Yang, Yueying Jiao, Qi Cui
Abstract
Abstract Traditional oxygen‐dependent photodynamic therapy (PDT) faces formidable challenges owing to abnormal angiogenesis and insufficient oxygen supply in solid tumors. Herein, a multifunctional nanoplatform (HRP‐DPBF‐Zn) consisting of photosensitizer zinc (II) phthalocyanine (ZnPc), singlet oxygen ( 1 O 2 ) probe 1,3‐diphenylisobenzofuran (DPBF), and hydrogen peroxide (H 2 O 2 ) catalyst horseradish peroxidase (HRP) is developed. Such HRP‐DPBF‐Zn nanosystems are expected to possess catalase‐like capacity for catalyzing intracellular H 2 O 2 to produce oxygen (O 2 ). The enhanced O 2 content further facilitates the production of cytotoxic 1 O 2 by ZnPc under laser irradiation. DPBF loading endows the nanoplatform with the high 1 O 2 sensitivity, which can promote precise PDT with fluorescent 1 O 2 feedback. HRP‐DPBF‐Zn exhibits excellent biocompatibility, efficient 1 O 2 generation, high 1 O 2 sensitivity, favorable H 2 O 2 ‐to‐O 2 decomposition, and superior fluorescence imaging properties. Both in vitro and in vivo experimental results confirm that such HRP‐DPBF‐Zn can accomplish highly specific and efficient PDT against hypoxic tumors, which shows great potential in tumor theranostics.