Photoisomerization-enhanced 1,3-dipolar cycloaddition of carbon-bridged octocyclic azobenzene with photo-released nitrile imine for peptide stapling and imaging in live cells
Jiajie Deng, Xueting Wu, Guiling Guo, Xiaohu Zhao, Zhipeng Yu
Abstract
). The CBOA-based photo-click reaction was proved to be bioorthogonal. In addition, the NoxaB peptide was successfully cross-linked by a CBOA stapler which plays a dual role: photo-control of the conformation of the peptide and photo-conjugation of probes in live cells.
Topics & Concepts
AzobenzeneNitrileCycloadditionPhotoisomerizationDouble bondIsomerizationChemistryIminePhotochemistryPeptide bondPeptidePolymer chemistryMoleculeOrganic chemistryCatalysisBiochemistryClick Chemistry and ApplicationsPhotochromic and Fluorescence ChemistryChemical Synthesis and Analysis