Generation of integration-free induced pluripotent stem cell lines from four pediatric ADHD patients
Leoni Grossmann, Cristine Marie Yde Ohki, Christian Döring, Per Hoffmann, Stefan Herms, Anna Maria Werling, Susanne Walitza, Edna Grünblatt
Abstract
Human induced pluripotent stem cell (iPSC) lines have been derived from four male patients with childhood attention-deficit hyperactivity disorder (ADHD). Children and adolescents between the ages 6 and 18 suffering from ADHD were recruited for this work. Isolated keratinocytes or peripheral blood mononuclear cells from the participants were reprogrammed into iPSCs using non-integrating Sendai virus to deliver the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc.
Topics & Concepts
Induced pluripotent stem cellReprogrammingSOX2KLF4BiologySendai virusPeripheral blood mononuclear cellAttention deficit hyperactivity disorderNeurodevelopmental disorderCellImmunologyEmbryonic stem cellGeneticsVirusPsychiatryIn vitroMedicineGeneAutism Spectrum Disorder ResearchPluripotent Stem Cells ResearchCRISPR and Genetic Engineering