Switching enzyme activity by a temperature responsive inhibitor modified polymer
Falko O. Rottke, Marie-Victoria Heyne, Stefan Reinicke
Abstract
A thermoresponsive NIPAAm-based polymer is combined with the selective acetylcholinesterase inhibitor tacrine in order to create a strict in sense on/off switch for enzymatic activity. This polymer-inhibitor conjugate inhibits AChE at room temperature and enables reactivation of AChE by heating above the cloud point of the conjugate.
Topics & Concepts
PolymerEnzymeChemistryChemical engineeringMaterials scienceBiophysicsPolymer chemistryOrganic chemistryBiologyEngineeringCholinesterase and Neurodegenerative DiseasesEnzyme Catalysis and ImmobilizationElectrochemical sensors and biosensors