Engineered 3D vessel-on-chip using hiPSC-derived endothelial- and vascular smooth muscle cells
Marc Vila Cuenca, Amy Cochrane, Francijna E. van den Hil, Antoine A.F. de Vries, Saskia A.J. Lesnik Oberstein, Christine L. Mummery, Valeria V. Orlova
Abstract
release kinetics in VSMCs could be quantified using automated image analysis based on open-source software CellProfiler and ImageJ on widefield or confocal images, setting the stage for use of the platform to study vascular (patho)physiology and therapy.
Topics & Concepts
Vascular smooth muscleBiologyCell biologyCrosstalkMural cellIntracellularCell typeInduced pluripotent stem cellPericyteCellEndothelial stem cellNeuroscienceAnatomySmooth muscleBiochemistryEndocrinologyEmbryonic stem cellIn vitroGeneOpticsPhysics3D Printing in Biomedical ResearchCellular Mechanics and InteractionsCell Image Analysis Techniques