Uptake, Stability, and Activity of Antisense Anti-<i>acpP</i> PNA-Peptide Conjugates in <i>Escherichia coli</i> and the Role of SbmA
Niloofar Yavari, Lise Goltermann, Peter E. Nielsen
Abstract
-PNA is ascribed to peptide degradation in the bacterial medium and in periplasmic space. Finally, the results show that stability and metabolism (by bacterial proteases/peptidases) should be taken into consideration upon design and activity/uptake analysis of BPPs (and antimicrobial peptides).
Topics & Concepts
Periplasmic spacePeptideEscherichia coliBiochemistryTripeptideBacteriaCytoplasmPeptide nucleic acidBacterial outer membraneBiologyChemistryGeneGeneticsAdvanced biosensing and bioanalysis techniquesBacteriophages and microbial interactionsRNA and protein synthesis mechanisms