Epitope Mapping of Polyclonal Antibodies by Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS)
Susanne Ständer, Laura R. Grauslund, Maria Scarselli, Nathalie Norais, Kasper D. Rand
Abstract
vaccine antigen. We identify four immunogenic regions located on the N- and C-terminal region of fHbp and provide insights into the relative abundance and avidity of epitope binding Abs present in the sample. Overall, our results show that HDX-MS can provide a unique and relatively fast method for revealing the binding impact of the entire set of pAbs present in blood samples after vaccination. Such information provides a rare view into effective immunity and can guide the design of improved vaccines against viruses or bacteria.
Topics & Concepts
ChemistryHydrogen–deuterium exchangeMass spectrometryPolyclonal antibodiesEpitope mappingEpitopeDeuteriumChromatographyAntibodyBiochemistryPeptide sequenceAtomic physicsGeneBiologyImmunologyPhysicsBacterial Infections and VaccinesRNA and protein synthesis mechanismsBacteriophages and microbial interactions