Cell-surface glycosaminoglycans regulate the cellular uptake of charged polystyrene nanoparticles
Paulo H. Olivieri, Marcelo Bispo de Jesus, Helena B. Nader, Giselle Z. Justo, Alioscka A. Sousa
Abstract
clathrin-mediated endocytosis and macropinocytosis. Upon the enzymatic shedding of surface GAGs, the uptake of anionic PS NPs increases while that of cationic PS NPs is dramatically reduced. Interestingly, the diminished uptake of cationic PS NPs is observed only when heparan sulfate, but not chondroitin sulfate, is cleaved from the cell surface. Heparan sulfate therefore serves as anchors/first receptors to facilitate the cellular entry of cationic PS NPs. These findings contribute to advance the basic science of nanoparticle endocytosis while also having important implications for the use of engineered nanocarriers as intracellular drug-delivery systems.