METTL14 and FTO mediated m <sup>6</sup> A modification regulate PCV2 replication by affecting miR-30a-5p maturity
Chen Li, Xiaoyan Wu, Ying Yang, Jianli Shi, Shuo Wang, Jiaxin Li, Jiaxin Li, Yao Tian, Chang Liu, Hong Han, Shaojian Xu, Xianjie Han, Yingru Ma, Limei Zheng, Jun Li, Jun Li
Abstract
The epigenetic modification of the N6-methyladenosine (m6A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m6A modifications are increased in PK-15 cells after PCV2 infection. In particular, PCV2 infection could increase the expression of methyltransferase METTL14 and demethylase FTO. Moreover, interfering with METTL14 accumulation reduced the m6A methylation level and virus reproduction, whereas depleting the FTO demethylase enhanced the m6A methylation level and stimulated virus reproduction. Besides, we showed that METTL14 and FTO regulate PCV2 replication by affecting the process of miRNA maturity, especially the miRNA-30a-5p. Taken together, our results demonstrated that the m6A modification positively affects PCV2 replication and the role of m6A modification in the replication mechanism of the PCV2 virus provides a new idea for the prevention and control of the PCV2.