Engineering protein theranostics using bio-orthogonal asparaginyl peptide ligases
Zhen Wang, Dingpeng Zhang, Xinya Hemu, Side Hu, Janet To, Xiaohong Zhang, Julien Lescar, James P. Tam, Chuan‐Fa Liu
Abstract
Bio-orthogonal tandem ligation using two asparaginyl peptide ligases with differential substrate specificities is a straightforward approach for the preparation of multifunctional protein biologics as potential theranostics.
Topics & Concepts
PeptideBiochemistryChemistryProtein engineeringPeptide libraryRecombinant DNAPeptide sequenceEnzymeGeneBiochemical and Structural CharacterizationMonoclonal and Polyclonal Antibodies ResearchGlycosylation and Glycoproteins Research